ABSTRACT
Aim: To compare the efficacy of dish wash solution, diluted lemon water, coconut oil and xylene as deparaffinizingagents for hematoxylin and eosin staining procedure.Objective: The objective is to find eco-friendly deparaffinizing agents like dish wash solution, diluted lemonwater and coconut oil as substitute to xylene and comparing the staining characteristics of each individualdeparaffinizing agent with Xylene.Materials and Methods: The study comprised of paraffin embedded 45 blocks of various tissues. Each block offour sections of 5 microns thickness was prepared. They were considered in four different groups like A, B, C andD. Tissue sections in Group A were stained with H & E method where xylene was used as deparaffinizing agent. Theother three sections were stained with H & E where dish wash solution, diluted lemon water and coconut oil wereused as deparaffinising agent’s alternative to Xylene. Staining characteristics were compared with xylene andscoring was given. The total score of 3–5 was regarded as satisfactory for diagnosis and less than that isinsufficient for diagnosis.Statitistical Analysis: Chi square test was used.Results: Adequacy of staining characteristics such as nuclear, cytoplasm, uniformity, clarity and crispiness ofstaining for diagnosis was greater with dish wash solution followed by diluted lemon water, coconut oil andxylene.Conclusion: The Eco-Friendly deparaffinizing agents such as dish wash solution, diluted lemon water, and coconutoil can be used as alternatives to xylene
ABSTRACT
Context: Gender identification is one of the most important aspects in the forensic science. It is based onscientific principles involving dental records, fingerprints and DNA comparisons. Sometimes, it becomes necessaryto apply easy techniques like cheiloscopy. The wrinkles and grooves on the labial mucosa form a characteristicpattern called lip prints, the study of which is referred to as cheiloscopy.Aim: The present study was done to evaluate the accuracy of various methods of lip prints in gender determinationand its stability over a period of time.Subjects and Methods: It consists of 80 healthy individuals (40 males and 40 females) in the age group of 18 to25 years. Lip prints were taken by lipstick, latent and digital photography methods. Data was collected by twoobservers at 0 month and 6months to assess their stability in gender determination.Statistical Analysis Used: Chi-square test was used.Results: The results obtained showed an accuracy of 0% and 57.5% by observer I, 30% and 67.5% by observer IIat 0 and 6th month in gender determination. The measurement of agreement in stability of lip prints was found tobe 27.8% by observer II.It was found that digital photography being the accurate method followed by lipstick andlatent methods.Conclusion: This study revealed that lip prints can be used for determining the gender of an individual.